hpi2 pe cy7 Search Results


hpi2 pe cy7  (Novus Biologicals)
94
Novus Biologicals hpi2 pe cy7
RNA-seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells <t>(HPi2+GFP+NTPDase3+)</t> from control and HNF1AKD pseudoislets for downstream RNA sequencing. (B) HNF1A transcripts per million (TPM) in sequenced samples. (C) Differential expression (DE) analysis revealed significantly up- and down-regulated genes after HNF1AKD in β cells; thresholds: Fold change (FC)= 1.5, adjusted P -value= 0.05. (D) Heatmap of DEGs in β cells after HNF1AKD. Significantly (E) downregulated and (F) upregulated gene ontology (GO) pathways and (G) downregulated KEGG pathways in HNF1AKD relative to control β cells. P = Benjamini-Hochberg adjusted P -value; all P <0.05.
Hpi2 Pe Cy7, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hpi2 pe cy7/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
hpi2 pe cy7 - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
hpi2 antibody (hic1-2b4.2b) [pe/cy7]  (Novus Biologicals)
N/A
The HPi2 Antibody (HIC1-2B4.2B) [PE/Cy7] from Novus is a HPi2 antibody to HPi2. This antibody reacts with Human, Mouse. The HPi2 antibody has been validated for the following applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen.
   Buy from Supplier

Image Search Results


RNA-seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells (HPi2+GFP+NTPDase3+) from control and HNF1AKD pseudoislets for downstream RNA sequencing. (B) HNF1A transcripts per million (TPM) in sequenced samples. (C) Differential expression (DE) analysis revealed significantly up- and down-regulated genes after HNF1AKD in β cells; thresholds: Fold change (FC)= 1.5, adjusted P -value= 0.05. (D) Heatmap of DEGs in β cells after HNF1AKD. Significantly (E) downregulated and (F) upregulated gene ontology (GO) pathways and (G) downregulated KEGG pathways in HNF1AKD relative to control β cells. P = Benjamini-Hochberg adjusted P -value; all P <0.05.

Journal: bioRxiv

Article Title: HNF1α transcriptional activation and repression maintain human islet α and β cell function

doi: 10.1101/2022.09.25.509394

Figure Lengend Snippet: RNA-seq of HNF1AKD β cells shows that HNF1α regulates insulin secretion, metabolism, developmental pathways, and cell-cell signaling in β cells. (A) Schematic of FACS scheme for isolation of transduced live β cells (HPi2+GFP+NTPDase3+) from control and HNF1AKD pseudoislets for downstream RNA sequencing. (B) HNF1A transcripts per million (TPM) in sequenced samples. (C) Differential expression (DE) analysis revealed significantly up- and down-regulated genes after HNF1AKD in β cells; thresholds: Fold change (FC)= 1.5, adjusted P -value= 0.05. (D) Heatmap of DEGs in β cells after HNF1AKD. Significantly (E) downregulated and (F) upregulated gene ontology (GO) pathways and (G) downregulated KEGG pathways in HNF1AKD relative to control β cells. P = Benjamini-Hochberg adjusted P -value; all P <0.05.

Article Snippet: Then, cells were stained with the following primary-secondary conjugated antibodies: HPi2-PE/Cy7 (Novus Biologicals NBP1-18946PECY7), NTPDase3-647 (clone hN3-B3S; Jean Sévigny Lab) and CD26-PE (BioLegend 302706).

Techniques: RNA Sequencing, Isolation, Control, Quantitative Proteomics

RNA-seq of HNF1AKD α cells identifies dysregulation of calcium channel complexes and ATPase-coupled transmembrane transport, as well as hormone secretion pathways shared with β cells. (A) Schematic of methods for isolation of transduced α cells (HPi2+GFP+CD26+) from control and HNF1AKD pseudoislets for downstream RNA sequencing; (i) brightfield and (ii) blue light (488nm) images of human HNF1AKD pseudoislets, scale bars=1000μm. (B) HNF1A transcripts per million (TPM) in sequenced α cell samples. (C) Differential expression (DE) analysis revealed significantly up- and down-regulated genes after HNF1AKD in α cells; thresholds: FC= 1.5, adjusted P -value= 0.05. (D) Venn diagram comparing α versus β cell DEGs revealed shared and cell-specific consequences of HNF1AKD. Gene ontology (GO) pathways of (E) shared and (F) α cell enriched DEG sets. (G) Boxplots displaying TPM of select DEGs. P = Benjamini-Hochberg adjusted P -value; * P <0.05; all P <0.05 for GO pathways.

Journal: bioRxiv

Article Title: HNF1α transcriptional activation and repression maintain human islet α and β cell function

doi: 10.1101/2022.09.25.509394

Figure Lengend Snippet: RNA-seq of HNF1AKD α cells identifies dysregulation of calcium channel complexes and ATPase-coupled transmembrane transport, as well as hormone secretion pathways shared with β cells. (A) Schematic of methods for isolation of transduced α cells (HPi2+GFP+CD26+) from control and HNF1AKD pseudoislets for downstream RNA sequencing; (i) brightfield and (ii) blue light (488nm) images of human HNF1AKD pseudoislets, scale bars=1000μm. (B) HNF1A transcripts per million (TPM) in sequenced α cell samples. (C) Differential expression (DE) analysis revealed significantly up- and down-regulated genes after HNF1AKD in α cells; thresholds: FC= 1.5, adjusted P -value= 0.05. (D) Venn diagram comparing α versus β cell DEGs revealed shared and cell-specific consequences of HNF1AKD. Gene ontology (GO) pathways of (E) shared and (F) α cell enriched DEG sets. (G) Boxplots displaying TPM of select DEGs. P = Benjamini-Hochberg adjusted P -value; * P <0.05; all P <0.05 for GO pathways.

Article Snippet: Then, cells were stained with the following primary-secondary conjugated antibodies: HPi2-PE/Cy7 (Novus Biologicals NBP1-18946PECY7), NTPDase3-647 (clone hN3-B3S; Jean Sévigny Lab) and CD26-PE (BioLegend 302706).

Techniques: RNA Sequencing, Isolation, Control, Quantitative Proteomics